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What is a Biocabinet speaks to Biocabinet performance tests and Biocabinet startup procedures.
Follow these start-up procedures when preparing for biocabinet use.
Turn off UV lights if in use and ensure that the sash is in the appropriate position.
Turn on fluorescent light and cabinet blower, if off.
Check the air intake and exhaust grilles for obstructions.
If the cabinet is equipped with an alarm, test the alarm and switch it to the "on" position.
Confirm inward airflow by holding a tissue at the middle of the edge of the viewing panel and ensuring that it is drawn in.
Disinfect the interior surfaces with a suitable, noncorrosive disinfectant.
Assemble all materials required for the procedure and load them into the cabinet; do not obstruct the air grilles; the working surface may be lined with absorbent paper with plastic backing; segregate "clean" items from "contaminated" items.
Wait 5 minutes to purge airborne contaminants from the work area. Follow these procedures for working in the cabinet :
Befor biocabinet use, don protective clothing and gloves as appropriate.
Perform operations as far to the rear of the work area as possible.
Avoid movement of materials or excessive movement of hands and arms through the front access opening during use; when you do enter or exit the cabinet, do so from straight on; allow the cabinet to stabilize before resuming work.
Keep discarded, contaminated material to the rear of the cabinet; do not discard materials in containers outside of the cabinet.
Do not work with open flames inside the cabinet.
If there is a spill during use, surface decontaminate all objects in the cabinet; disinfect the working area of the cabinet while it is still in operation (do not turn the cabinet off).
Follow these procedures upon completion of biocabinet use :
Allow the cabinet to run for 5 minutes with no activity.
Close or cover open containers before removing them from the cabinet.
Surface disinfect objects in contact with contaminated material before removal from the cabinet.
Remove contaminated gloves and dispose of them as appropriate; wash hands.
Don clean gloves, and ensure that all materials are placed into biohazard bags within the cabinet.
Using a suitable non-corrosive disinfectant (e.g., 70% ethanol), disinfect interior surfaces of cabinet; periodically remove the work surface and disinfect the area beneath it (including the catch pan) and wipe the surface of the UV light with disinfectant.
Turn off the fluorescent light and cabinet blower when appropriate (some cabinets must be left on at all times; if you are unsure, check with your cabinet certifier, safety officer or building maintenance personnel).
Turn on the UV light if appropriate (do not turn on when people are working close by); UV must be tested to ensure that it is emitting a germicidal wavelength (ask your cabinet certifier to perform this test).
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